Firstly the property which belonged to your grandmother is not ancestral property in nature.
It becomes her own and absolute property.
If the previous Will written by her was cancelled and new Will was written by a registered document, then the latest Will only shall be legally valid instrument.
The beneficiary of the latest Will are the only person/s entitled to acquire the properties bequeathed in the Will, if the other persons were left out by the testator without allotting any share in any property, then it cannot be claimed a s right by the dissatisfied members of the same family because it was the absolute property of the testator and she had full rights to dispose the properties in any manner as per desire and wish.
However if there is a suspicion about any fraud or acts of forgery then the Will can be challenged by the other siblings.
Certain elements, such as, a shaky and doubtful signature, a feeble or uncertain mind of the testator, unfair disposition of property, unjust exclusion of legal heirs, and the active involvement of the major beneficiary in the execution of the will, are indications of suspicious circumstances.
If you have any slightest indications of any of the above mentioned items, you can challenge the Will.