Hindrance will come into effect when she proceeds with the sale. There is no legal remedy to stop anyone not to knock the door of law whether he is entitled or not. It is a matter of trial. The sale of property take time and there is enough time for the brother to challenge the Will in the court and take stay. Now, the whole process of sale would be in doldrum. Earnest money taken would be sought back, etc. etc.
Will is not required to be registered. However, if the witnesses are in favour of mother, they can get the case decided in favour of mother.
But the thing is how to come out of it. If your mother enter into sale agreement with any genuine buyer, your brother definitely go to the court. You have to go through the process of law and the thing is there should be no problem to your mother and the buyer.
In my opinion, you may create a bogus buyer and enter into sale agreement on the face of your brother and instigate him to go to the court of law. Once the case is initiated, it would definitely bring result and thereafter, there would be no problem in selling the property. In litigation patience is required. Except this, I do not find any other way which is permanent and effective. Discuss with your mother. Good luck.