You have sent a notice for partition and they have replied with baseless allegations, some of which has hurt your sentiments, right?
For filing a defamation case u/s 499/500, some ingredients are essential:
You should have proof to prove that they were wrong allegations and was intended to spoil your reputation in the society as well as to defame your name and fame in the society;
How did it affect you and what are all the losses you have suffered (mental)
other issues as mentioned in the provision of the law referred.
You may apprise a good lawyer about the defamatory contents in the reply notice and can arrange to issue a notice to the people who offended you (not the lawyer who issued notice under his banner), wait for the reply or compliance of the demand made in the legal notice, after that you can proceed with a criminal private complaint before the jurisdictional magistrate court.