Father Died - Registered Will Executed on my Name for Mutation

Sir, My Father & his four brothers divided their Ancestral Land property 18 Yrs ago. I am the Single Son & Two sisters of my father. Father & Brothers constructed a commercial complex as per Partitioned Deed As My father won't have any income, I expended nearly 15 Lakhs for the purpose of construction for our share of partition. Due to my consistant efforts and responsibilities towards family my father wrote & Registered a will providing all rights to me after his death. Recently my father died. I executed the Will and applied for mutation. Muncipal authorities made mutation in my name after taking declaration from me and No Objection affidavit from my mother. --------- Surprisingly my sisters demanding for their rights on ancestral property. Please Clarify my doubts 1. Is my married sisters (married before 2000 yr) will got property rights excluding my father's Registered will..? 2. If they claim for Ancestral Land property... How they get Land property rights after partition of Joint property by my father and his brother...? 3. After construction of Commercial building Jointly how they get property rights on Joint Property..? 4. As the entire five families got more than 20 successors..Is any possibility to get property rights to my sisters... Please Calrify....